Figure 1.

HES Suppression of IL-33

(A) IL-33 levels (ELISA) in supernatants of naive murine lung cells (1 × 10⁵ per well), cultured for 1 hr with Alternaria (Alt) allergen (200 μg/ml) and HES (10 μg/ml).

(B) Propidium iodide (PI) and annexin V (AnnV) staining of cells from (A) was used to assess apoptosis (PI–AnnV+) versus necrosis (PI+AnnV+).

(C) IL-33 levels (ELISA) in supernatants of naive murine lung cells, freeze-thawed in the presence of HES.

All data shows SEM of 2–3 replicates, and are representative of 2–3 repeat experiments. Error bars show SEM.