Fig. 1.

Chromatogram, which presents the final separation of all seven compounds postulated as (bio)degradation products applied at a concentration of 100 μM and introduced to the 14-day-old post-cultured Bg11 medium. Amine compounds, in the form of tosyl derivatives, were separated in the following order: 1—AMPA; 2—2-AEP; 3—NPMG; 4—MAMPA; 5—Gly; 6—NMA; 7—SAR. The chromatogram also contains the conditions of separation marked as a dotted line. The lower one corresponds to the flow rate, while the upper one presents time-dependent participation of a KH₂PO₄ buffer in a mobile phase composition, with respect to MeCN. The unnumbered peak with a retention time of approximately 6 min is attributed to p-toluenesulfonyl chloride