Table 6.
Concentrations of (bio)degradation products of aminopolyphosphonates after 2 weeks of culturing with cyanobacteria
| Substrates | Cyanobacteria strain | Medium | Concentration of postulated products (μM) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| AMPA | 2-AEP | NPMG | MAMPA | Gly | NMA | SAR | |||
| ATMP | Anabaena 007 | Bg11 | 115.0 ± 1.7 | – | – | 3.2 ± 0.5 | – | – | – |
| Bg11-P | 102.3 ± 8.5 | – | – | 2.4 ± 0.4 | – | – | – | ||
| Chroococcidiopsis 049 | Bg11 | 41.3 ± 0.9 | – | – | 2.8 ± 0.2 | – | – | – | |
| Bg11-P | 126.8 ± 2.5 | – | – | 2.5 ± 0.3 | – | – | – | ||
| Chroococcus 055 | Bg11 | 85.8 ± 7.6 | – | – | 2.9 ± 0.4 | – | – | – | |
| Bg11-P | 90.8 ± 2.8 | – | – | 3.6 ± 0.2 | – | – | – | ||
| Fischerella 067 | Bg11 | 133.4 ± 12.4 | – | – | 3.4 ± 0.6 | – | – | – | |
| Bg11-P | 111.4 ± 4.3 | – | – | 3.9 ± 1.5 | – | – | – | ||
| Nostoc 129 | Bg11 | 72.0 ± 5.6 | – | – | 3.8 ± 1.0 | – | – | – | |
| Bg11-P | 109.7 ± 6.1 | – | – | 3.3 ± 1.4 | – | – | – | ||
| GBMP | Anabaena 007 | Bg11 | 4.7 ± 0.5 | – | 1.8 ± 0.3 | 111.5 ± 9.2 | – | – | – |
| Bg11-P | 5.4 ± 0.3 | – | 1.2 ± 0.1 | 67.3 ± 1.8 | – | – | – | ||
| Chroococcidiopsis 049 | Bg11 | 8.3 ± 1.1 | – | 2.3 ± 0.3 | 155.8 ± 9.8 | – | – | – | |
| Bg11-P | 3.7 ± 1.0 | – | 1.0 0.3 | 63.3 ± 8.5 | – | – | – | ||
| Chroococcus 055 | Bg11 | 5.2 ± 0.3 | – | 1.5 ± 0.3 | 82.8 ± 6.8 | – | – | – | |
| Bg11-P | 3.0 ± 1.0 | – | 1.0 ± 0.1 | 51.5 ± 1.2 | – | – | – | ||
| Fischerella 067 | Bg11 | 6.2 ± 0.6 | – | 2.3 ± 0.2 | 108.6 ± 1.2 | – | – | – | |
| Bg11-P | 4.2 ± 0.1 | – | 1.2 ± 0.1 | 65.7 ± 0.7 | – | – | – | ||
| Nostoc 129 | Bg11 | 9.5 ± 0.4 | – | 2.0 ± 0.2 | 114.5 ± 2.3 | 1.5 ± 0.1 | 1.1 ± 0.1 | – | |
| Bg11-P | 5.8 ± 0.2 | – | 1.2 ± 0.1 | 73.2 ± 3.4 | – | – | – | ||
| HDTMP | Anabaena 007 | Bg11 | a | – | – | – | – | – | – |
| Bg11-P | – | – | – | – | – | – | – | ||
| Chroococcidiopsis 049 | Bg11 | 1.8 ± 0.2 | – | – | 0.7 ± 0.1 | – | – | – | |
| Bg11-P | – | – | – | – | – | – | – | ||
| Chroococcus 055 | Bg11 | 2.1 ± 0.3 | – | – | 0.5 ± 0.1 | – | – | – | |
| Bg11-P | – | – | – | – | – | – | – | ||
| Fischerella 067 | Bg11 | a | – | – | – | – | – | – | |
| Bg11-P | – | – | – | – | – | – | – | ||
| Nostoc 129 | Bg11 | 2.5 ± 0.1 | – | – | – | – | – | – | |
| Bg11-P | – | – | – | – | – | – | – | ||
| DTPMP | Anabaena 007 | Bg11 | 2.2 ± 0.2 | – | – | – | – | – | – |
| Bg11-P | 1.8 ± 0.1 | – | – | – | – | – | – | ||
| Chroococcidiopsis 049 | Bg11 | 1.2 ± 0.0 | – | – | 0.5 ± 0.0 | – | – | – | |
| Bg11-P | a | – | – | – | – | – | – | ||
| Chroococcus 055 | Bg11 | 4.0 ± 0.1 | – | a | 1.1 ± 0.0 | – | – | – | |
| Bg11-P | – | – | – | – | – | – | – | ||
| Fischerella 067 | Bg11 | 14.5 ± 1.0 | – | – | 2.0 ± 0.1 | – | – | – | |
| Bg11-P | 2.3 ± 0.2 | – | – | 1.1 ± 0.0 | – | – | – | ||
| Nostoc 129 | Bg11 | 9.0 ± 0.7 | – | – | 1.1 ± 0.2 | – | – | – | |
| Bg11-P | – | – | – | – | – | – | – | ||
The impact of the lack of inorganic phosphate on the accessibility of AAPs for microbes was determined (via the Bg11-P medium application). Concentrations are expressed in micromoles per liter (μM) + S.D. (n ≥ 3). Initially, all media were fortified with the addition of aminopolyphosphonates at a final concentration of 100 μM. The presented values were determined in a post-cultured media after 14 days of cyanobacteria culturing using the elaborate HPLC method
–Is attributed to not detected
aThe presence of an intermediate was confirmed in the experimental culture but it did not exist in a 14-day post-culture media; “0.0”—S.D. value below 0.05 μM