Table 3.
Medium for determination of number of soil microorganisms
| Microorganisms | Medium | References |
|---|---|---|
| Olig—oligotrophic bacteria Oligp—oligotrophic spore-forming bacteria |
Peptone 0.10 g; meat extract 0.10 g; NaCl 0.05 g; agar 10.0 g; H2O 1.0 dm3; pH 7.0–7.2 | Ohta and Hattori (1983) |
| Cop—copiotrophic bacteria Copp—copiotrophic spore-forming bacteria |
Peptone 10 g; meat extract 10 g; NaCl 5 g; agar 10.0 g; H2O 1.0 dm3; pH 7.0–7.2 | Ohta and Hattori (1983) |
| Az—Azotobacter | K2HPO4 1.5 g; MgSO4⋅7H2O 0.3 g; NaCl 0.3 g; FeSO4⋅7H2O 0.005 g; MnSO4⋅7H2O 0.005 g; CaCO3 3.0 g; saccharose 15.0 g; agar 7.0 g; H2O 1.0 dm3; pH 7.0–7.2 | Fenglerowa (1965) |
| Art—Arthrobacter | CaH2PO4 0.25 g; K2HPO4 1.0 g; MgSO4⋅7H2O 0.25 g; glycerol 10 cm3; agar 14.0 g; H2O 1.0 dm3; pH 7.0 | Mulder and Antheumisse (1963) |
| Ps—Pseudomonas | Peptone 20 g; K2HPO4 1.5 g; MgSO4⋅7H2O 1.5 g; agar 14.0 g; glycerol 10 cm3; H2O 1.0 dm3; pH 7.2 | Mulder and Antheumisse (1963) |
| Am—ammonifying bacteria | Peptone 5 g; K2HPO4 0.5 g; MgSO4 0.2 g; NaCl 0.2 g; MnSO4 0.005 g; FeSO4 0.005 g; NH4)2Fe(SO4)2⋅6H2O 0.005 g; agar 14 g; nystatin 7.5 mg·1 dm−3 medium; H2O 1.0 dm3; pH 7.0–7.2 | Winogradski (1953) |
| Im—nitrogen immobilisation bacteria | K2HPO4 0.5 g; MgSO4 0.2 g; NaCl 0.2 g; MnSO4 0.005 g; FeSO4 0.005 g; (NH4)2Fe(SO4)2⋅6H2O 0.005 g; saccharose 0 g; NH4NO3 0.2 g; agar 14 g; H2O 1.0 dm3; pH 7.0–7.2 | Winogradski (1953), our modification—instead of (NH4)2SO4 used NH4NO3 |
| Cel—cellulolytic bacteria | K2HPO4 0.5 g; MgSO4 0.2 g; NaCl 0.2 g; MnSO4 0.005 g; FeSO4 0.005 g; KNO3 1.0 g; (NH4)2Fe(SO4)2⋅6H2O 0.005 g; agar 14 g; H2O 1.0 dm3; pH 7.0–7.2 | Winogradski (1953) |
| Act—actinomycetes | Soluble starch 10.0 g; casein 0.3 g; KNO3 2.0 g; NaCl 2.0 g; K2HPO4 2.0 g; MgSO4⋅7H2O 0.05 g; CaCO3 0.02 g; FeSO4 0.01 g; agar 20.0 g; H2O 1 dm3; 50 cm3 aqueous solution of nystatin 0.05%; 50 cm3 aqueous solution of actidione 0.05%; pH 7.0 | Parkinson et al. (1971) |
| Fun—fungi | Peptone 5 g; K2HPO4 1.0 g; glucose 10 g; MgSO4⋅7H2O 0.5 g; agar 20.0 g; H2O 1 dm3; 3.3 cm3 aqueous solution of bengal rose 1%; 25 cm3 aqueous solution of aureomycin 0.01%; pH 5.9 | Martin (1950) |