Table 4.
Methods of determination of soil enzyme activity
| Enzyme | Substrate | Product/unit | References |
|---|---|---|---|
| Deh—dehydrogenases (EC 1.1) | 2,3,5-Triphenyl tetrazolium chloride (TTC) | Triphenyl fomazan (TFF), μmol kg−1 DM of soil h−1 | Öhlinger (1996) |
| Cat—catalase (EC 1.11.1.6) | H2O2—aqueous solution | O2, mol kg−1 DM of soil h−1 | Alef and Nannipieri (1998) |
| Ure—urease (EC 3.5.1.5) | Urea—aqueous solution | N-NH4, mmol kg−1 DM of soil h−1 | Alef and Nannipieri (1998) |
| Glu—β-glucosidase (EC 3.2.1.21) | 4-Nitrophenyl-β-D-glucopyranoside (PNG) | 4-Nitrophenol (PN), mmol kg−1 DM of soil h−1 | Alef and Nannipieri (1998) |
| Pac—acid phosphatase (EC 3.1.3.2) | Disodium 4-nitrophenyl phosphate hexahydrate (PNP) | 4-Nitrophenol (PN), mmol kg−1 DM of soil h−1 | Alef and Nannipieri (1998) |
| Pal—alkaline phosphatase (EC 3.1.3.1) | Disodium 4-nitrophenyl phosphate hexahydrate (PNP) | 4-Nitrophenol (PN), mmol kg−1 DM of soil h−1 | Alef and Nannipieri (1998) |
| Aryl—aryosulphatase (EC 3.1.6.1) | Potassium-4-nitrophenylsulfate (PNS) | 4-Nitrophenol (PN), mmol kg−1 DM of soil h−1 | Alef and Nannipieri (1998) |