Figure 4.

Morphologic alteration of embryonic and early postnatal cortex in WT and Manf^-/- mice. A–J, Coronal sections of the developing cortex stained with Nissl. A, B, There is no difference in the overall appearance of the cortex between WT and Manf^-/- mice at E13.5. C, D, At E15.5, the IZ and CP are thinner in the Manf^-/- mice than in WT littermates, but the thickness of SVZ/VZ is not changed. E, F, At E18.5, the CP is thinner in Manf^-/- mice compared to WT mice. G–J, At P1 and P7, the cortex layer is thinner, prominently at Layer II–V in Manf^-/- mice compared to controls. K, At E18.5, the cell density in the CP is higher in the Manf^-/- mice compared to WT mice (blue, DAPI). L, At P7, DAPI+ nuclei in the cortex Layer II–IV is higher in Manf^-/- mice compared to WT mice. M, There was no difference in VZ/SVZ thickness between WT and Manf^-/- embryos or pups at any time point studied. N, The thickness of IZ was significantly reduced at E15.5 in the Manf^-/-, but recovered by E18.5 (L, *p < 0.05, Student’s t test). O, The thickness of PP/CP was significantly reduced at E15.5, E18.5, and P1 in Manf^-/- compared to WT littermates (M, ***p < 0.001, **p < 0.001, *p < 0.05, respectively, Student’s t test). P, The thickness of cortical layers was measured at P7, and we observed significantly thinner Layers II/IV and V in Manf^-/- mice compared to WT littermates (***p < 0.001, Student’s t test). Q, Quantification of the DAPI+ nuclei; scale bars: 100 µm.