Figure 5.

Loss of MANF does not affect the proliferation or survival of neural precursors. A, C, Analysis of S-phase progenitor cells by BrdU pulse labeling of WT and Manf^-/- embryos at E13.5, E15.5, and P1. A similar number of S-phase cells are in the Manf^-/- cortex relative to the WT littermates. The average number of BrdU-labeled cells in the SVZ was calculated at E13.5, E15.5, and P1. B, D, The number of pH3-positive cells in the VZ/SVZ of E15.5 Manf^-/- mice is not different from WT littermates. The number of PAX6-positive cells (E, F) or Tbr2-positive cells (G, H) in the cortex did not differ between WT and Manf^-/- brains. I, J, Very few apoptotic cells were found in cerebral cortex from E15.5 WT as well as Manf^-/- mice immunostained with activated caspase-3. Cell nuclei were counterstained with DAPI (blue). Data are expressed as the mean ± SEM, n = 3-4; scale bars: 50 µm (A) and 100 µm (B, E, G, I).