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. 2017 Sep 29;4(5):ENEURO.0214-17.2017. doi: 10.1523/ENEURO.0214-17.2017

Figure 6.

Figure 6.

Distribution of cortical layer markers CTIP2, TBR1, and Cux1. A, The number of CTIP2+ cells at E15.5 are similar between WT and Manf-/- in CP. At E18.5, there is a significant increase in CTIP2+ neurons density at deep layers of CP in the Manf-/- compared to the WT littermates, but there is no difference at P1 and P7. B, Tbr1 is located at the lower CP in the WT controls and Manf-/- mice at E15.5. In the Manf-/- brain, there is a significant decrease in the number of Tbr1+ neurons in the deep layer of CP at E18.5 compared to WT controls, but there is a full recovery by P1 and P7. C, At E15.5, Cux1 is located at the VZ/SVZ, but is absent in the CP in WT and Manf-/- brain. There are fewer Cux1+ neurons in the superficial CP at E18.5 and Layers II–IV at P1 of Manf-/- brain than in WT controls. In the Manf-/- mouse brain, more Cux1-positive neurons remained in the IZ at E18.5 and in the Layers V and VI at P1. At P7, there is no difference in Cux1-positive neurons in the cortex between the genotypes. D, Representative photomicrographs of E18.5 CP stained for Tbr1 (red), CTIP2 (green), and nuclei (DAPI, blue). E, Representative photomicrographs of E18.5 CP stained for Cux1 (red), CTIP2 (green), and nuclei (DAPI, blue). D, Representative photomicrographs of P7 cortex stained for Tbr1 (red), CTIP2 (green), and nuclei (DAPI, blue). G, The number of CTIP2+ cells in the CP at E15.5 and E18.5 and Layer V at P1 and P7 (n = 3; **p < 0.01). H, The number of Tbr1+ neurons in the CP at E15.5 and E18.5 and at Layer VI at P1 and P7 (n = 3; ***p < 0.001). I, The number of Cux1+ neurons in the CP at E18.5; Layers II–VI, V–VI at P1 and P7 (n = 3; **p < 0.01, ***p < 0.001); scale bar: 100 µm.