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. 2017 Sep 22;6(8):700–707. doi: 10.1530/EC-17-0209

Figure 3.

Figure 3

RDM1 expression examined by qRT-PCR and Western blot. CT is the threshold cycle for target amplification in K1 cells. ΔCT is equal to the difference in threshold cycles for the target and reference; ΔΔCT calculates the relative quantification of target. (A) RDM1 mRNA expression level in K1 and TPC1 cells. ΔCT = CTRDM1 − CTGAPDH. (B) RDM1 protein in K1and TPC1 cells. Compared with shCtrl, the level of RDM1 protein in K1 and TPC cells decreased after the RDM1 expression was silenced by RNAi. GAPDH is a control. The RDM1 and GAPDH proteins were observed as major bands corresponding to molecular weights of 26 and 34 kDa, respectively. (C) and (D) Knock down efficiency was determined by qRT-PCR. The RDM1 mRNA expression level in K1 cells after infection with different lentiviruses was measured using the 2−ΔΔCT method. ΔΔCT = mean (CTRDM1/CTGAPDH) − CTRDM1. 2−ΔΔCT of shRDM1 and shCtrl in K1 and TPC1 cells were 0.5 ± 0.1 vs 1.0 ± 0.0, 0.3 ± 0.1 vs 0.9 ± 0.0, respectively (**P < 0.01).