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. 2017 Oct 24;7:13936. doi: 10.1038/s41598-017-13698-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tissue distribution in LLC bearing C57BL mice. C57BL mice were inoculated i.m. on the right flank with LLC cells (2 × 10⁶). After 10 days, HydroCuP was administered i.p. at a dose of 50 mg/kg. The mice were sacrificed after 24 hours and tumor, brain, spleen, kidney, intestine, liver and stomach were excised. Tissues were washed in ice-cold saline and weighed after removing excess fluid. All samples were mineralized in HNO₃ and Cu content in each sample was measured by GF-AAS. Error bars indicate the S.D. **P < 0.01; *P < 0.05 (tumor vs organs).