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. 2017 Oct 20;8:2043. doi: 10.3389/fmicb.2017.02043

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Copyright © 2017 Minniti, Hagen, Porcellato, Jørgensen, Pope and Vaaje-Kolstad.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic illustration of the experimental design. A total of 45 fish were used in this experiment. Fifteen fish were sampled directly from the main tank (Tank_1) and skin mucus samples were collected (T0). The resting 30 fish, after netting, were transferred into Tank_2 and Tank_3, and successively mucus samples were collected at 3 h (T3) and 24 h (T24) post-handling. In parallel, water samples were collected at different time points, with 3 replicates taken per tank for each time point. Rearing water from Tank_2 and Tank_3 was additionally sampled before fish were transferred into the new tanks (Ctrl).