Table 2.
Analytical sensitivity in a background of unmethylated DNA, determined in three independent experiments, each in triplicate
| Proportion of methylated DNA in 20 ng input DNA | Amount of methylated DNA | ASTN1 | DLX1 | ITGA4 | RXFP3 | SOX17 | ZNF671 | ACHE | IDS |
|---|---|---|---|---|---|---|---|---|---|
| 10% | 2 ng | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 |
| 5% | 1 ng | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 |
| 2% | 0.4 ng | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 |
| 1% | 0.2 ng | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 8/9 |
| 0.5% | 0.1 ng | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 8/9 |
| 0.2% | 0.04 ng | 9/9 | 9/9 | 9/9 | 9/9 | 8/9 | 9/9 | 9/9 | 7/9 |
For all six markers and IDS, unmethylated, bisulfite-converted DNA was taken as background DNA for all primer pairs