Table 1.
Estimation of total lipid yield of the mutants using Nile red fluorimetry
| Treatment | Mutant | Total lipid yield (g L−1) |
|---|---|---|
| MNNG | YlB6 | 0.24 ± 0.01 |
| YIC4 | 0.19 ± 0.01 | |
| YIC5 | 0.24 ± 0.01 | |
| YIC6 | 0.27 ± 0.01 | |
| YIC7 | 0.30 ± 0.02 | |
| YIB2 | 0.19 ± 0.01 | |
| MNNG + cerulenin | YIB3 | 0.18 ± 0.01 |
| YID5 | 0.27 ± 0.02 | |
| YIE1 | 0.39 ± 0.02 | |
| YIE2 | 0.20 ± 0.01 | |
| WT | 0.20 ± 0.01 |
The mutants were grown on LAM containing 30 g L−1 glucose for 96 h. The values represent the mean ± SD of three independent determinations