Table 2.
Biomass, total lipid yield, yield coefficient, volumetric productivities of biomass and lipid of mutants
| Treatment | Mutant | X (g L−1) | L (g L−1) | YL/X (g g−1) | QX (g L−1 h−1) | QL(g L−1 h−1) |
|---|---|---|---|---|---|---|
| No treatment | WT | 7.1 ± 0.03a | 3.2 ± 0.03a | 0.45 ± 0.004a,d | 0.074 ± 0.0002a | 0.033 ± 0.0002a |
| YIB6 | 10.86 ± 0.01b | 5.97 ± 0.01b | 0.55 ± 0.001b | 0.113 ± 0.0001b | 0.062 ± 0.0001b | |
| MNNG | YIC4 | 5.76 ± 0.03c | 2.73 ± 0.03c | 0.47 ± 0.005c,d | 0.059 ± 0.0003c | 0.028 ± 0.0003c |
| YIC5 | 5.91 ± 0.02d | 2.74 ± 0.02c | 0.46 ± 0.004d | 0.062 ± 0.0002d | 0.028 ± 0.0002c | |
| YIC6 | 6.25 ± 0.03e | 2.23 ± 0.03d | 0.35 ± 0.005e | 0.065 ± 0.0002e | 0.023 ± 0.0003d | |
| YIC7 | 7.1 ± 0.03a | 4.28 ± 0.01e | 0.60 ± 0.004f | 0.073 ± 0.0002a | 0.044 ± 0.0002e | |
| YIB2 | 7.0 ± 0.03a | 3.7 ± 0.02f | 0.53 ± 0.006g | 0.072 ± 0.0006a | 0.038 ± 0.0001f | |
| YIB3 | 5.40 ± 0.03f | 1.92 ± 0.03g | 0.35 ± 0.006e | 0.056 ± 0.0003f | 0.019 ± 0.0003g | |
| MNNG + cerulenin | YID5 | 5.73 ± 0.01c | 2.42 ± 0.02h | 0.42 ± 0.006h | 0.059 ± 0.0001c | 0.025 ± 0.0002h |
| YIE1 | 5.84 ± 0.03d | 3.91 ± 0.01i | 0.67 ± 0.004i | 0.061 ± 0.0003d | 0.041 ± 0.0001i | |
| YIE2 | 4.4 ± 0.03g | 1.57 ± 0.02j | 0.35 ± 0.004e | 0.045 ± 0.0002g | 0.016 ± 0.0002j |
The values indicate the mean ± standard deviation (n = 3). Mean values within a column with different superscript letters (a,b,c,d,e,f,g,h,i,j) differ significantly and were determined statistically using Graph Pad InStat ver 3.10 software (One-way ANOVA, Tukey–Kramer multiple comparison test, p < 0.05). Separate analysis was done for each column. The wild type and mutants were grown on LAM containing 100 g L−1 WCO for 96 h. WT wild type, X biomass, L total lipid yield, Y L/X lipid/biomass yield coefficient, Q X volumetric biomass productivity, Q L volumetric lipid productivity. All yields were determined gravimetrically