Table 3.
Comparison of fatty acid methyl ester profile of the mutants with the wild type
| Fatty acid methyl ester | (wt % of total fatty acid methyl esters) | |||
|---|---|---|---|---|
| YlB6 | YIC7 | YIE1 | WTa | |
| Caprylic acid methyl ester (C8:0) | ND | ND | ND | 25.0 |
| Lauric acid methyl ester (C12:0) | ND | ND | ND | 3.2 |
| Myristic acid methyl ester (C14:0) | ND | ND | ND | 1.7 |
| Palmitic acid methyl ester (C16:0) | 29.1 | 28.1 | 30.4 | 21.1 |
| Stearic acid methyl ester (C18:0) | 6.3 | 6.1 | 8.5 | 3.4 |
| Arachidic acid methyl ester (C20:0) | 9.8 | 2.7 | 8.7 | ND |
| Heneicosanoic acid methyl ester (C21:0) | 8.9 | 2.9 | 10.5 | 1.8 |
| Total of fatty acids: Saturated | 54.1 | 39.8 | 58.1 | 56.2 |
| Palmitoleic acid methyl ester (C16:1) | ND | 4.1 | 10.0 | 0.9 |
| cis-10-Heptadecanoic acid (C17:1) | ND | ND | ND | 8.0 |
| Oleic acid methyl ester (C18:1n9c) | 33.6 | 40.1 | 25.4 | 21.0 |
| cis-11Eicosanoic acid (C20:1) | ND | ND | ND | 2.0 |
| Total of fatty acids: Monounsaturated | 33.6 | 44.2 | 35.3 | 31.9 |
| Linoleic acid methyl ester (C18:2n6c) | 12.3 | 16.0 | 6.6 | 11.8 |
| Total of fatty acids: Polyunsaturated | 12.3 | 16.0 | 6.6 | 11.8 |
| Total of fatty acids | 100 | 100 | 100 | 99.98 |
The wild type and mutants were grown on LAM containing 100 g L−1 WCO. The values represent the mean ± SD of three independent determinations
WT Wild type; aas reported earlier [9], ND Not detected