Table 4.
Fuel properties of biodiesel produced by the mutants and wild type grown on WCO
| Property/Test | Results for Y. lipolytica NCIM 3589 | US biodiesel standards ASTM D6751 | European biodiesel standards EN14214 | Indian biodiesel standards IS15607 | |||
|---|---|---|---|---|---|---|---|
| YlB6 | YIC7 | YIE1 | WT | ||||
| Density at 25 °C (g cm−3)a | 1.07 (0.87) | 1.07 (0.86) | 1.08 (0.88) | 1.04 (0.87) | NS | 0.86-0.90 | 0.86-0.90 |
| Water content (vol%)a | ND | ND | ND | ND | 0.05 max | 0.25 max | 0.03 max |
| TAN (mg NaOH g-1)a | 2 | 2 | 2 | 2.8 | 0.8 max | 0.5 max | 0.5 max |
| FFA (%)a | 1 | 1 | 1 | 1.4 | NS | NS | NS |
| Cu strip corrosiona | Class 1a | Class 1a | Class 1a | Class 1a | Class 3 max | Class 1 max | Class 1 max |
| CNc | 66.7 | 59.43 | 68.6 | 50.8 | 47-65 | 51 min | 51 min |
| Kinematic viscosity (40 °C; mm2 s−1)c | 5.07 | 4.57 | 5.09 | 3.6 | 1.9-6.0 | 3.5-5.0 | 3.5-5.0 |
| SNa | 190.38 (194.65) | 197.6(193.75) | 197.4 (192.28) | 256.16 (249.4) | NS | NS | NS |
| IVa | 50.03 (47.9) | 69.4 (65.82) | 50.2 (42.58) | 37.8 (47.9) | NS | 120max | NS |
| HHV(M J kg−1)b | 40.87 | 40.49 | 40.9 | 36.77 | NS | NS | NS |
| Concentration of γ-linolenic acid (C18:3) (%)a | 0 | 0 | 0 | 0 | NS | 12max | NS |
| FAME having ≥ 4 double bonds (%)a | ND | ND | ND | ND | NS | 1 max | NS |
The wild type and mutants were grown on LAM containing 100 g L−1 WCO. The experimental values are the mean ± SD of three independent determinations
WT Wild type, as reported earlier [9]; ND Not Detected, NS Not Specified
aExperimentally determined values, followed by predicted ones, if any, in brackets
bCalculated using predicted values of SN and IV
cPredicted values as mentioned in Materials and methods [23]