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. Author manuscript; available in PMC: 2018 Oct 17.
Published in final edited form as: Anal Chem. 2017 Sep 25;89(20):10687–10695. doi: 10.1021/acs.analchem.7b01121

Figure 2.

Figure 2

Schematic representation of peptide-level PLIMSTEX workflow. Protein (cyan) is first incubated with a solution containing increasing concentration of ligand (blue). Deuterium buffer (red) is then mixed and incubation occurs for a fixed time (tfix). HDX is quenched and the protein is submitted to protease digestion. Centroid deuterium incorporation for the responsive peptide is decreased from unbound to bound state. Binding affinity is extracted from fitting of PLIMSTEX curve that is obtained by plotting HDX extent against ligand concentration.