Figure 5.

Effect of puerarin on the expression of p-Akt (Ser473) in SW1353 cells. SW1353 cells were treated with different concentrations of puerarin (0–300 µM) for 48 h. Protein expression of p-Akt and Akt were detected in the (A) presence or (B) absence of the phosphoinositide 3-kinase inhibitor LY294002. The relative protein expression was quantified and assessed, respectively. A representative blot is presented for each experiment. Data are presented as the mean ± standard deviation (n=4). *P<0.05, **P<0.01 and ***P<0.001, compared with the untreated control. Akt, RAC-alpha serine/threonine-protein kinase; p, phosphorylated.