Figure 5.

AMPK activation is involved in autophagy and suppression of viability of K562 cells in response to adenine. Cells were (A) treated with adenine alone or (B) pretreated with AMPK inhibitor dm (5 µM) for 2 h followed by treatment with adenine for 24 h, and then subjected to western blot analysis. GAPDH was used as internal control. The apparent molecular weights of the detected proteins are indicated. Quantitative data are expressed as the mean ± standard error of the mean following three independent experiments. (C) A cell viability assay was performed to investigate the effects of dm on K564 cells. *P<0.05 and **P<0.01 as compared with the untreated control; ^#P<0.05 and ^##P<0.01 as compared with the 4.0 mM adenine treatment group. AMPK, AMP-activated protein kinase; p, phosphorylated; mTOR, mechanistic target of rapamycin; Atg5, autophagy protein 5; dm, dorsomorphin; C, control.