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. Author manuscript; available in PMC: 2018 Aug 4.
Published in final edited form as: J Proteome Res. 2017 Jul 17;16(8):2983–2992. doi: 10.1021/acs.jproteome.7b00275

Figure 1.

Figure 1

Feasibility of antibody-based enrichment strategy. The tryptic peptides of in vitro nitrated BSA were subjected for anti-tyrosine antibody IP before LC-MS/MS analysis. (A) The overlap of identified nitrotyrosine peptides between replicates. (B) The enrichment of NT-containing peptides by anti-nitrotyrosine antibody. The number on each column are the number of PSM of nitrotyrosine peptides (grey) and non-nitrated peptides (black) identified from each sample. PSM, peptide spectra matched.