Table 1.
The nitrotyrosine-containing BSA peptides identified by LC-MS/MS analysis of tryptic digest of nitrated BSA (input) and the IP fraction (from anti-nitrotyrosine antibody enrichment).
| Sequence | # of PSMs
|
Nitrotyrosine sites | |
|---|---|---|---|
| Input | IP | ||
| MPCTEDYLSLILNR | 12 | 7 | Y7(Nitro) |
| DDPHACYSTVFDK | 8 | 13 | Y7(Nitro) |
| RPCFSALTPDETYVPK | 10 | 6 | Y13(Nitro) |
| DAFLGSFLYEYSR | 2 | 2 | Y11(Nitro) |
| ETYGDMADCCEK | 3 | 20 | Y3(Nitro) |
| EYEATLEECCAK | 3 | 23 | Y2(Nitro) |
| EYEATLEECCAKDDPHACYSTVFDK | 0 | 1 | Y2(Nitro) |
| GLVLIAFSQYLQQCPFDEHVK | 1 | 0 | Y10(Nitro) |
| HPEYAVSVLLR | 1 | 5 | Y4(Nitro) |
| HPYFYAPELLYYANK | 0 | 10 | Y11(Nitro) |
| HPYFYAPELLYYANK | 0 | 8 | Y11(Nitro); Y12(Nitro) |
| HPYFYAPELLYYANK | 0 | 2 | Y3(Nitro); Y5(Nitro); Y11(Nitro) |
| HPYFYAPELLYYANK | 7 | 0 | Y5(Nitro) |
| LGEYGFQNALIVR | 0 | 4 | Y4(Nitro) |
| RHPEYAVSVLLR | 4 | 13 | Y5(Nitro) |
| RHPYFYAPELLYYANK | 0 | 1 | Y6(Nitro); Y12(Nitro) |
| RHPYFYAPELLYYANK | 1 | 0 | Y6(Nitro) |
| YICDNQDTISSK | 3 | 21 | Y1(Nitro) |
| YIYEIAR | 4 | 52 | Y1(Nitro); Y3(Nitro) |
| YIYEIAR | 38 | 236 | Y3(Nitro) or Y1 (Nitro) |
| YNGVFQECCQAEDK | 0 | 10 | Y1(Nitro) |
PSM, peptide spectrum match; IP, immunoprecipitation.