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. Author manuscript; available in PMC: 2017 Oct 25.
Published in final edited form as: Nature. 2016 Oct 31;540(7631):139–143. doi: 10.1038/nature20555

Figure 4. Dynamin-2 is required for STS-induced mitochondrial fragmentation.

Figure 4

a, Representative images of TOM20 immunofluorescence in n = 41/42, 45/43, 45/43 scrambled, Drp1, Dyn2 siRNA cells (without/with 1.5 hour STS treatment), respectively. Scale bars = 10 μm. b, Mitochondrial morphology was quantitated for mean area per mitochondria, and mean mitochondria per ROI.. Error bars represent s.e.m.. *p<0.01 statistical significance calculated by ANOVA and obtained from three biological replicate experiments c, Cytochrome C release and Bax recruitment were evaluated by immuno-blot of cytosol/membrane fractions. a-Tubulin verified cytosolic fractions and TOM20 verified membrane fractions. d, Drp1 and Dyn2 depletion were confirmed by immuno-blot of post-nuclear supernatants (PNS).