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. 2017 Oct 24;7:13892. doi: 10.1038/s41598-017-14108-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Neighbor-joining phylogenetic tree constructed based on partial pol genes from MSM who were newly diagnosed in 2013 in Zhejiang. The scale bar indicates 5% nucleotide sequence divergence. Values on the branches represent the percentage of 1000 bootstrap replicates, and bootstrap values over 70% are shown in the tree. The CRF01_AE cluster and the CRF07_BC cluster are compressed into a triangle on the left and expanded to show details on the right. The reference sequences obtained from the Los Alamos HIV database are marked as solid circles. The CRF01_AE reference sequences include Ref.01_AE.CN.05.05GX001.GU564221 and Ref.01_AE.TH.90.CM240.U54771. The CRF07_BC reference sequences include Ref.07_BC.CN.05.XJDC6431_2.EF368372, Ref.07_BC.CN.05.XJDC6441.EF368370, Ref.07_BC.CN.98.98CN009.AF286230 and Ref.07_BC.CN.97.97CN001.AF286226. Sequences with present address of Hangzhou are indicated by hollow upward-pointing triangle (△). URFs are labeled with solid downward-pointing triangles (▼), and their sample ID is indicated. The sequences with SDRMs are marked as five-pointed stars (★). The previously reported 01B strain with accession number KR823980 is labeled with a solid upward-pointing triangle (▲).