Table 5.
Histological features on liver biopsies
| RBC 1 | RBC 2 | RBC 3 | RBC 4 | RBC 5 | HBOC 1 | HBOC 2 | HBOC 3 | HBOC 4 | HBOC 5 | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Designated viability | Nonviable | Viable | Nonviable | Viable | Viable | Nonviable | Nonviable | Viable | Viable | Viable | |
| Large droplet steatosis1 (%) | 0 | <1 | 15 | <5 | 0 | 80 | 0 | 0 | <1 | 0 | |
| Small droplet steatosis2 (%) | 0 | 10 | 40 | 20 | 5 | 70 | <1 | 1 | 80 | 0 | |
| Glycogen depletion3 (pre-NMP-L/post-NMP-L) |
- | 95/10 | 90/80 | 90/10 | 80/15 | 90/85 | 60/65 | 10/10-15 | 80/50 | 60/15 | |
| Detached hepatocytes4 (%) (pre-NMP-L/post-NMP-L) |
- | 1 / 2 | 20 / 15 | - | 0 / 1 | 0/5 | 0/50 | 0/0 | 0/0 | 0/0 | |
| Coagulative Necrosis5 (%) (pre-NMP-L/post-NMP-L) |
- | 0 / 5 | 0 / 5 | 0 / 30 | 0 / 0 | 0/1 | 0/2 | 0/0 | 0/0 | 0/0 | |
| Other finding | Hepatitis with severe cholestasis | Patchy congestion | Steatohepatitis | Congested, did not flush well | |||||||
Abbreviations:
HBOC, Hemopure group; NMP-L, normothermic machine perfusion – Liver ; RBC, Red blood cell group
Note: Values designated with “–“ are missing; Steatosis determined in the pre-NMP-L biopsy
Large droplet macrovesicular steatosis is defined as a single large fat droplet within the hepatocyte cytoplasm displacing the nucleus. Values are % of hepatocytes containing fat
Small droplet macrovesicular steatosis is defined as fat droplets, usually multiple within the cytoplasm of the hepatocyte which do not displace the nucleus. Values are % of hepatocytes containing fat
Glycogen depletion is graded as % of hepatocytes which do not contain glycogen.
Detached hepatocytes are the % of hepatocytes which have lost cohesion from each other and from the sinusoidal lining
Necrosis is depicted as the percent of total hepatocytes in the biopsy which show classical ischemic-type coagulative necrosis.