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. 2017 Mar 6;1(4):370–384. doi: 10.1210/js.2016-1071

Figure 4.

Figure 4.

FOXA transcription factors stimulate ACE2 expression. (a) 832/13 cells were transfected with luciferase reporters containing the wild-type human −454/−1 ACE2 promoter sequence or the promoter sequence in which the FOXA motif was mutated. Cells were cotransfected with expression plasmids for FOXA1, FOXA2, FOXA3, or a control plasmid. Luciferase activities were determined 44 hours after transfection and corrected for the background activities of empty pGL3-Basic. *P < 0.05, **P < 0.01 vs control. (b) 832/13 cells were transfected with expression plasmids for forkhead box transcription factors or a control plasmid. RNA was isolated 22 hours after transfection. The content of ACE2 mRNA relative to β-actin mRNA was determined. ***P < 0.001 vs control. (c) 832/13 cells were transfected with luciferase reporters containing the wild-type human −454/−1 ACE2 promoter sequence or the promoter sequence in which the FOXA motif was mutated. Transfected cells were treated with 0.5 mM AICAR or dimethyl sulfoxide (DMSO) vehicle as control for 44 hours. Luciferase activities were corrected for the background activities of empty pGL3-Basic. *P < 0.05, ***P < 0.001 vs control. (d) 832/13 cells were treated with 0.5 mM AICAR or DMSO vehicle as control for 48 hours. The content of ACE2 mRNA relative to β-actin mRNA was determined.