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. Author manuscript; available in PMC: 2017 Oct 25.
Published in final edited form as: Methods Mol Biol. 2014;1213:365–377. doi: 10.1007/978-1-4939-1453-1_30

Fig. 3.

Fig. 3

Murine iPS cell-derived Treg cells were stimulated with plate-coated anti-CD3/anti-CD28 mAbs. Intracellular cytokine production was analyzed by flow cytometry after gating on live CD4+ CD25+ cells (dark lines, shaded areas indicate isotype controls). (a) Intracellular TGF-β and IL-10. (b) Intracellular IL-2 and IFN-γ