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. 2017 Oct 13;13(10):e1007053. doi: 10.1371/journal.pgen.1007053

Fig 8. BAK1 phosphorylation increases in roots of Arabidopsis after AtRALF1 treatment in a dose-dependent manner.

Fig 8

pBAK1:BAK1-6xHA plants were treated with water (H20), 2 μM RALF1(9–49) [R9-49], 0.1 μM BL, 1 and 2 μM AtRALF1 for 20 min. After treatment, the microsomal fraction was isolated from roots. BAK1 protein was immunoprecipitated with anti-HA beads and subjected to immunoblot analysis with anti-phospho-Thr and anti-HA antibodies. The numbers indicate the relative intensity of the bands.