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. 2017 Oct 19;12:7709–7720. doi: 10.2147/IJN.S148001

Figure 2.

Figure 2

Rat ovariectomy model and BMMSC identification.

Notes: (A) Two-dimensional images and three-dimensional reconstructed images of the OVX rat model 3 months postsurgery obtained by micro-CT analysis. (B) The quantitative data obtained by micro-CT analysis of BV/TV. (CD) Characterization of rat BMMSCs. The BMMSCs expressed positive staining of the cells with alizarin red (C) or Oil Red O (D), which demonstrated the osteogenic and adipogenic differentiation potential of the BMMSCs. Magnification: ×400. (E) The BMMSCs were positive for MSC markers, including CD29 and CD90, and negative for hematopoietic and endothelial markers, including CD34 and CD45. *P<0.05.

Abbreviations: BMMSCs, bone marrow mesenchymal stem cells; BV/TV, bone volume per total volume; OVX, ovariectomy.