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. 2017 Oct 19;12:7709–7720. doi: 10.2147/IJN.S148001

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© 2017 Xing et al. This work is published and licensed by Dove Medical Press Limited

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Cell proliferation and osteogenesis-related gene expression.

Notes: (A) Cell proliferation of samples after 1, 4, and 7 days of incubation measured by the CellTiter-Blue Cell Viability Assay. (B) Gene expression, including ALP, COL-1, RUNX2, and OCN in primary osteoblasts cultured on titanium surfaces 3, 7, and 14 days post-incubation. Data were generated by real-time PCR and are expressed in mean ± SD relative to GAPDH. *P<0.05 vs TNS; ^#P<0.05 vs TNS-PEM; ^&P<0.05 vs TNS-PEM-IGF1-50.

Abbreviations: PEM, polyelectrolyte multilayer; TNS, titania nanosheet; TNS-PEM, specimens without IGF1; TNS-PEM-IGF1-50, specimens with 50 ng/mL of IGF1; TNS-PEM-IGF1-100, specimens with 100 ng/mL of IGF1; ALP, alkaline phosphatase.