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. 2017 Oct 19;12:7709–7720. doi: 10.2147/IJN.S148001

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© 2017 Xing et al. This work is published and licensed by Dove Medical Press Limited

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ALP staining, activity and ECM mineralization.

Notes: (A) ALP staining of MSCs after 7 and 14 days culture. (B) ECM mineralization on different samples after 21 days culture of BMMSCs. (C) ALP activity of MSCs on different samples cultured for 1 and 2 weeks. (D) Quantitative results of calcium deposition. *P<0.05 vs TNS; ^#P<0.05 vs TNS-PEM; ^&P<0.05 vs TNS-PEM-IGF1-50.

Abbreviations: ALP, alkaline phosphatase; BMMSCs, bone marrow mesenchymal stem cells; PEM, polyelectrolyte multilayer; TNS, titania nanosheet; TNS-PEM, specimens without IGF1; TNS-PEM-IGF1-50, specimens with 50 ng/mL of IGF1; TNS-PEM-IGF1-100, specimens with 100 ng/mL of IGF1.