Table 1.
The primers for the target
| Gene | Sequence | Pair |
|---|---|---|
| GAPDH forward | AGTTCAACGGCACAGTCAAGG | 113bp |
| GAPDH reverse | AGCACCAGCATCACCCCAT | |
| ALP forward | CTGCTGATCACTCCCACGTT | 113bp |
| ALP reverse | GATGGCTGTGAAGGGCTTCT | |
| COL-1a1 forward | AAAGGCTGGAGAACGAGGTG | 131bp |
| COL-1a1 reverse | CGGGACCTTGTTCACCTCTC | |
| Runx2 forward | GCGCATTCCTCATCCCAGTA | 138bp |
| Runx2 reverse | GGTGGGGAGGATTGTGTCTG | |
| OCN forward | GAATAGACTCCGGCGCTACC | 135bp |
| OCN reverse | TCCTGGAAGCCAATGTGGTC |
Note: The primers for the target and housekeeping genes used in the real-time PCR and the amplicon size (base pairs) of the resulting PCR products.
Abbreviations: ALP, alkaline phosphatase; COL-1, type 1 collagen; OCN, osteocalcin; Runx2, runt-related transcription factor 2.