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Effect of CK-666 on filopodia number and length. (A) The average number of filopodia per cell and (B) the length of filopodia was quantitated using image analysis software (Bitplane) from TM cells treated with DMSO vehicle control (n = 27 cells, 496 filopodia counted) and CK-666 (n = 23 cells; 231 filopodia counted). Data are from three biological replicates. The gray diamond in each box is the mean value. * P < 0.001 by ANOVA with Bonferroni correction.
