Table 1.

Genetic tools for adventitial fibroblasts

Perivascular Expression Profile							Expression in other cell types
Mouse line	JAX #	Tissue	Cell Type	Uninjured	Injury/model	Reference
Collagen1a1-GFP	n/a	Ascending aorta	Adventitial fibroblast	E, P	n/a	35	Interstitial cardiac fibroblasts35, 102, 103 Activated HSC68, 121, 122 Embryonic/postnatal HSC8, 68 Interstitial lung123 and kidney58 cells Podocytes58 Osteoblasts71 Colon fibroblast124 Spinal cord perivascular fibroblasts73
		Pulmonary vein	Adventitial fibroblast	E, P	n/a	35
		Heart	Adventitial fibroblast	E, P	TAC	35
		Heart	Adventitial fibroblast	A	mdx mice	66
		Liver	PF	n/a	CCl4, BDL	121, 122
		Liver	PF	E, P	CCl4	68
		Liver	PF	P	n/a	8
		Kidney	Perivascular fibroblast	A	UUO	58
		Skeletal muscle	Fibro-adipogenic precursors	A	mdx mice	36
Enolase2-Cre	006663	Ascending aorta	Adventitia	A	Ang II	75	Neural cells75
FSP/S1004A-Cre	012641	Ascending aorta	Adventitial fibroblast	A	Ang II	75	Liver Kupffer and macrophages80
Gli1CreERT2	007913	Ascending aorta	Adventitial MSC-like pericytes	A	ApoE−/− mice HFD & CKD	59, 83	Neural stem cells85 Cranial sutures84 Hair follicle stem cells86 Lung mesothelial cells87 Lung peribronchial and perivascular smooth muscle cells88
		Femoral artery	Adventitial MSC-like pericytes	A	Wire injury	83
		Heart	Adventitial MSC-like pericytes	A	Ang II, TAC	59
		Liver	Adventitial MSC-like pericytes	A	CCl4	59
		Lung	Adventitial MSC-like pericytes	A	Bleomycin	59
		Kidney	Adventitial MSC-like pericytes	A	UUO, IRI	59
Patched-1lacZ	003081	Aortic root/thoracic aorta	Adventitia	P	n/a	44	Lung mesothelial cells87 Hair follicle stem cells86 Neural tube cells90 Kidney interstitial, epithelial, glomerular, and endothelial cells23, 94 Duodenal mesenchymal cells95 Lymphatic EC96
		Heart	Adventitia	P	n/a	44
		Pulmonary trunk	Adventitia	P	n/a	44
		Intercostal artery	Adventitia	P	n/a	44
		Mesenteric artery	Adventitia	P	n/a	44
		Femoral arteries	Adventitia	P	n/a	44
Patched-2lacZ	005827	Aortic root/thoracic aorta	Adventitia	P	n/a	44
		Heart	Adventitia	P	n/a	44
		Pulmonary trunk	Adventitia	P	n/a	44
		Intercostal artery	Adventitia	P	n/a	44
		Mesenteric artery	Adventitia	P	n/a	44
		Femoral artery	Adventitia	P	n/a	44
PDGFRα-CreER	018280	Skeletal muscle	Perivascular cell	A	CTX	36	Glial progenitors105
PDGFRαnGFP	007669	Thoracic aorta	Adventitial cell	A	n/a	66	Interstitial cardiac fibroblasts35, 101–103 HSC100 Lung lipofibroblast98, 99 Dermal fibroblasts97 Oligodendrocytes108, 109 Astrocytes106 Neural stem cells107 Perichondrium104 Adipocytes precursors110
		Heart	Adventitial cell	A	n/a	66
		Skeletal muscle	Fibro-adipogenic precursors	A	CTX	36
		Liver	PF	A	CCl4	100
Sca1-GFP	012634	Heart	Adventitia	A	mdx mice	66	EC66 HSC111
Tcf21lacZ	n/a	Heart	Adventitial fibroblast	E	n/a	102	Interstitial cardiac fibroblasts115 Kidney stroma118
		Aortic root	Adventitial fibroblast	A	ApoE−/− mice HFD	115
		Heart	Adventitial fibroblast	A	ApoE−/− mice HFD	115
Tcf21lacZ	n/a	Kidney	Adventitial cell	A	n/a	117	Kidney peritubular cells117 Kidney and lung stroma116
Tcf21mCrem	n/a	Heart	Adventitial fibroblast	E, P	n/a	102	Splenic119, lung, and liver8 interstitial cellsInterstitial cardiac fibroblasts102 Kidney podocytes and mesangial cells118
		Aortic root	Adventitial fibroblast	A	ApoE−/− mice HFD	115
		Heart	Adventitial fibroblast	A	ApoE−/− mice HFD	115
		Liver	Adventitial fibroblast	A	n/a	MDT, unpublished
		Lung	Adventitial fibroblast	A	n/a	MDT, unpublished
		Kidney	Adventitial fibroblast	A	n/a	MDT, unpublished

Abbreviations: A, adult; Ang II, angiotensin II; BaCl₂, barium dichloride; BDL, bile duct ligation; CCl₄, carbon tetrachloride; CKD, chronic kidney disease; CTX, cardiotoxin; E, embryonic; EC, endothelial cell; GFP, green fluorescent protein; HFD, high fat diet; HSC, hepatic stellate cell; IRI, ischemia reperfusion injury; MSC, mesenchymal stem cell; n/a, not available; P, postnatal; PF, portal fibroblast; TAC, transverse aortic constriction; UUO, unilateral ureteral obstruction