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Determination of KI and kinact for inactivation of CYP3A4 (midazolam) by licochalcone A. (A) Time- and concentration-dependent inactivation of CYP3A4 (midazolam) by licochalcone A. (B) Non-linear regression analysis of kobs,app. Activity is expressed as a percentage of remaining activity compared with the control containing no inhibitor. Experiments were carried out three times.
