Formulation and characterization of GW-encapsulated NPs. (A) Schematic of Col IV-GW-NP design. The lipids/polymer/drug mixture were dissolved in benzyl alcohol/ethyl acetate co-solvent (2:8, v/v), and the NPs were formed by modified single-emulsion method in water via a single self-assembly process. (B) The mean hydrodynamic diameters of empty NPs, GW-NPs, and Col IV-GW-NPs in PBS and serum (100% FBS), respectively, determined by DLS. (C) Representative TEM images of Col IV-GW-NPs. The scale bars of the left image and the right high-magnification image are 100 nm and 20 nm, respectively. (D)
In vitro drug release profiles of GW-NPs and Col IV-GW-NPs incubated at 100 rpm and 37 °C in PBS.