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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Adv Healthc Mater. 2017 Jul 21;6(20):10.1002/adhm.201700313. doi: 10.1002/adhm.201700313

Figure 6.

Figure 6

Effects of GW-NPs or Col IV-GW-NPs on hepatic mRNA expression in mice. Ldlr−/− mice (n=6 to 9/group) were fed a western diet for 14 weeks and then switched to a chow diet for another 5 weeks. While on the chow diet, the mice were intravenously injected twice a week with 8 mg/kg of body weight (based on the amount of free GW) of free GW, GW-NPs, or Col IV-GW-NPs or PBS (control) to study the effects of the different treatments on hepatic lipid expression. Total mRNA was isolated and retrotranscribed to determine the expression levels of selected genes. (A) Expression of the ATP-binding cassette A1 (ABCA1), (B) ATP-binding cassette G1 (ABCG1), (C) ATP-binding cassette G5 (ABCG5), (D) sterol regulatory element binding protein (SREBP-1c), (E) fatty acid synthase (FASN), and (F) cytochrome P450 7A1 (Cyp7a1). Cyclo A was used as a housekeeping control. (PBS, n=8; free GW, n=6; GW-NPs, n=9; Col IV-GW-NPs, n=7) (* p<0.05 and ** p<0.01). Data are expressed as mean ± SEM.