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. 2017 Oct 25;7:14051. doi: 10.1038/s41598-017-13622-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miR-204 up- and down-regulation in Human Islets. Scatter plot of ddPCR droplets’ fluorescence results from a representative experiment of miR-204 up- or down-regulation in transfected purified human islets. Vertical dotted lines separate treatment groups (panel a). Bar plot of mean miR-204 copies per ng of total RNA measured by ddPCR, according to treatment group. Individual results of 3 transfection replicates are shown for each islet preparation (squares = HP1260, circles = HP1266; diamonds = HP1269). Treatment groups are identified by bar color: untreated cells white bars, miR-204 down-regulation light grey bars, miR-204 up-regulation dark grey bars (panel b).