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. 2017 Oct 25;7:13987. doi: 10.1038/s41598-017-14410-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The decrease of RecA levels in Msm ΔpafBC is due to transcriptional downregulation of the coding gene and is not caused by increased proteolysis. (A) His₆-RecA was expressed in Msm wild type, Msm ΔpafBC or Msm ΔprcB carrying a plasmid encoding His ₆ -recA gene under the control of an acetamide-inducible promoter. Cells were subsequently washed and then transferred to non-inducing growth medium. Samples were taken at the indicated time-points and turn-over of His₆-RecA was analysed by an anti-His₆-tag immunoblot. Full-length blots are shown in Supplementary Figure S7. Equal loading was controlled by an anti-RpoB immunoblot. (B) Msm ΔpafBC exhibits reduced transcription of recA. Values are shown as mean ± SD and represent three biological replicates measured in triplicates. An unpaired two-tailed Student’s t-test was applied for statistical analysis; ****p value < 0.001.