Figure 7.

(A) DNA-damage induced upregulation of RecA is impaired in Msm ΔpafBC. Msm wild type, the pafBC deletion mutant and a complemented strain were grown in 7H9 to an OD₆₀₀ between 0.8 to 1.0 at 37 °C. Cultures were split, supplemented with mitomycin C (80 ng/ml) as indicated and were incubated for another 4 h. RecA levels of cell-free lysates were analysed by an anti-RecA immunoblot. Full-length blots are shown in Supplementary Fig. S8. To control for equal loading, an anti-RpoB immunoblot was performed. (B) Levels of PafBC do not alter under DNA damaging growth conditions. Samples of Msm wild type cells were prepared as described in (A) and were analysed by an anti-PafBC immunoblot.