Table 1.

Binding of DTG to PFV IN complexed with various DNA sequences.

Name of the double-stranded DNA	Sequence of the reactive stranda Pos: 21 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1	DTG-binding (%)b
		Fluorescence intensity procedurec	Fluorescence anisotropy procedured
DNA#1	5′-TAT ACA AAA TTC CAT GA C A AT−3′	100	100
DNA#2	5′-GGA ATA AAA TTC CAT GA C A AT−3′	83 ± 5	n.d.e
DNA#3	5′-GGA ATC TAA TTC CAT GA C A AT−3′	89 ± 6	96 ± 3
DNA#4	5′-GGA ATC TAG TTC CAT GA C A AT−3′	84 ± 4	n.d.
DNA#5	5′-GGA ATC TAG CTC CAT GA C A AT−3′	101 ± 5	n.d.
DNA#6	5′-GGA ATC TAG CGC CAT GA C A AT−3′	76 ± 5	n.d.
DNA#7	5′-GGA ATC TAG CGG CAT GA C A AT−3′	20 ± 3	42 ± 6
DNA#8	5′-GGA ATC TAG CGG CGC ATC A AT−3′	7 ± 2	8 ± 2
DNA#9	5′-GGA ATC TAG CGG CGC ATA GGT-3′	9 ± 2	15 ± 3
DNA#10	5′-TAT ACA AAA TTC CAT GAA GGT-3′	23 ± 3	n.d
DNA#11	5′-TAT ACA AAA TTC CAT GAA GAT−3′	24 ± 2	29 ± 3
DNA#12	5′-TAT ACA AAA TTC CAT GA C AGT-3′	72 ± 6	77 ± 4
DNA#13f	5′-TAT ACA AAA TTC CAT GA C A−3′	66 ± 4	79 ± 5

^aPFV nucleotides are in bold. The underlined sequence corresponds to the conserved retroviral dinucleotide sequence (CA) preceding the 3′-P cleavage site. ^bNormalized by the cognate sequence (DNA#1). All experiments were performed with stoichiometric concentrations of IN and DNA (1.8 μM each). ^cThe procedure is indicated in Fig. 2C–D legend. [DTG] = 0.3 μM; [Mg²⁺] = 1 mM. ^dThe procedure is indicated in Fig. 4A–B legend. [DTG] = 0.6 μM; [Mg²⁺] = 10 mM. ^eNon determined. ^f19-mer oligonucleotide for mimicking the processed DNA product (when hybridized with the complementary sequence of DNA#1). All percentages are mean values ± SD from three independent experiments.