Figure 3.

Effects of yucasin DF on wild-type and auxin overexpression plants. (A) Effects of YDF on high-auxin phenotypes resulting from YUC1, YUC2 and YUC6 overexpression. 35S::YUC1 plants were grown for 5 days in 1/2 MS medium in the presence of 20 µM YDF. pMDC7::YUC2 and pMDC7::YUC6 plants were cultured for 5 days in 1/2 MS medium in the presence of 5 µM estradiol (ER) and 20 µM YDF. The images were taken of representative phenotypes. Scale bar = 10 mm. (B) Effects of YDF on wild-type seedlings. The seedlings were grown vertically for 6 days on 1/2 MS agar plates with or without exogenous 10 nM IAA. Scale bar = 10 mm. (C) Inhibition of primary root growth by YDF in the presence or absence of 10 nM IAA. Root length was measured after 6 days of cultivation on vertical 1/2 MS agar plates. Values are the means ± S.D. (n = 30–35).