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. 2017 Oct 25;7:13992. doi: 10.1038/s41598-017-14332-w

Figure 5.

Figure 5

Effects of yucasin DF on the phenotypes of Arabidopsis auxin biosynthesis and signaling mutants. (A) Effects of YDF on root phenotypes of Arabidopsis auxin biosynthesis mutants. Arabidopsis seedlings were vertically grown for 6 days on 1/2 MS medium containing YDF. Primary root length of wild-type, cyp79b2 79b3, sav3-1/taa1 and yuc3 5 7 8 9 seedlings were measured. The relative root length was shown as the values (%) and a mock-treated root was adjusted to 100%. The actual length (mm) of mock-treated roots were indicated. Box-and-whisker plots show a median (centerline), upper/lower quartiles (box limits) and maximum/minimum (whiskers) n > 15. Statistical significance assessed by Welch’s two sample t-test. Asterisks indicate significant differences between WT and mutant at *P < 0.05 and **P < 0.01. (B) 6-d-old Arabidopsis wild-type, cyp79b2 79b3, sav3-1/taa1 and yuc3 5 7 8 9 seedlings grown vertically with 50 µM YDF. Scale bar = 10 mm. (C) Effects of YDF on shoot phenotypes of Arabidopsis auxin biosynthesis and signaling mutants. Arabidopsis wild-type, cyp79b2 79b3 and tir1 afb2 seedlings were cultured for 11 days on 1/2 MS plate containing TAA inhibitors [L-kynurenine (L-Kyn) and AONP], YUC inhibitor [YDF] and TIR1 antagonist [auxinole] with or without 0.5 μM IAA. The values in parentheses indicate concentration (µM). The petiole length of the first leaves was shown as relative value (100%) and the actual length (mm) of mock-treated plants. n > 18 Statistical significance assessed by Welch’s two sample t-test. *P < 0.05 and **P < 0.01.