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. 2017 Oct 25;7:13992. doi: 10.1038/s41598-017-14332-w

Figure 6.

Figure 6

Effects of yucasin analogs on YUC1 recombinant enzyme activity and endogenous IAA levels. (A) Recombinant AtYUC1-His enzyme activity. The enzymatic reaction was performed using 0.25 µg of AtYUC1-His enzyme and 20 µM IPA at 35 °C for 30 min. The inhibitor was added at 1 or 10 µM. Values are the means ± S.D. of three independent experiments. (B) 5-d-old wild-type seedlings were incubated with inhibitor in liquid 1/2 MS medium for 10 h. The seedlings (n = 5–8) were pooled for each sample, and three samples were analyzed for each data point. Endogenous IAA levels were measured using LC-MS/MS. Values are the means ± S.D.