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. 2017 Oct 25;7:13992. doi: 10.1038/s41598-017-14332-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chemical and metabolic stability of yucasin and fluorinated yucasin. (A) Chemical stability of the inhibitors in culture medium. The inhibitors (100 µM) were incubated in 1/2 MS liquid medium for 6 days at 24 °C under continuous light. The sample was analyzed using HPLC at regular intervals. The residual amount of inhibitor is shown as the relative value (%) compared with the initial amount. (B) Metabolic stability of the inhibitors in root cell lysate. The inhibitors (250 µM) in Arabidopsis root homogenate were incubated for 18 h at 24 °C in the dark. Residual inhibitors were measured using HPLC after the incubation. Values are the means ± S.D.