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. 2017 Oct 25;7:13979. doi: 10.1038/s41598-017-14277-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of leucine zipper, SKIK tag, and DsbC on mAb synthesis in CFPS. Rabbit Fab (anti-Listeria monocytogenes clone) was produced by CPFS with various combinations of the presence (+) or absence (−) of the LZ fusion, N-terminal SKIK tag, and DsbC. Oblique lines indicate negative controls without DNA template. (a) ELISA result for L. monocytogenes antigen and bovine serum albumin (BSA; negative control). The primary and secondary antibodies were three-fold diluted CFPS samples and 2000-times diluted anti-rabbit Ig(G + M)-horseradish peroxidase (HRP) conjugate. (b) Western blotting of 1 µL of CPFS product separated by SDS-PAGE. Hc and Lc were visualised with anti-HA tag-HRP and anti-FLAG tag-HRP conjugated antibodies, respectively. The intensities of each band quantified by image J software are indicated at the bottom. Full-length blots are included in Supplementary Information (Supplementary Fig. S4).