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. 2017 Oct 25;7:14058. doi: 10.1038/s41598-017-14565-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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No change in BK_Ca subunit total protein levels in arterial lysates from non-diabetic and diabetic patients. (A) Representative immunoreactive bands corresponding to BK_Ca α1 and BK_Ca β1 subunits in lysates from untransfected HEK293 cells (HEK), HEK293 cells transfected with either BK_Ca α1 or BK_Ca β1 subunit and human arteries from non-diabetic patients (n = 3 lysates per condition). Full-length blots are shown in Supplementary Fig. S2A. (B) Representative immunoreactive bands corresponding to BK_Ca α1 and BK_Ca β1 subunits, and β-actin as a normalization control. Full-length blots are shown in Supplementary Fig. S2B. (C) Corresponding densitometric summary data for each subunit obtained using arterial lysates from non-diabetic (n = 10 lysates) and diabetic (n = 10 lysates) patients. *P < 0.05, Mann-Whitney test. Significance was compared between non-diabetic and diabetic datasets.