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. 2017 Oct 25;7:14061. doi: 10.1038/s41598-017-14444-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of fracture healing 14 days after trauma. Areas of different tissues as determined by Safranin O staining: total callus area (a), relative cartilage content (b) and osseous tissue content (c). Representative images of Safranin O-stained femurs at day 14 (d). Scale bar 500 µm. Relative numbers of osteoblasts (e) and osteoclasts (f) in the periosteal fracture callus. Representative images of TRAP staining at day 14 (g). Scale bar 100 µm. Fx: mice with isolated fracture, Fx + TXT: mice with combined fracture and thoracic trauma. n = 5–6, *p < 0.05, **p < 0.01, ***p < 0.001.