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. 2017 Oct 25;7:13996. doi: 10.1038/s41598-017-14454-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of endogenous miR-193b increases DDAH1 mRNA and protein expression in MCF7 cells. (A) DDAH1 mRNA expression levels were assessed following transfection of 100 nM control inhibitor or targeting miR-193b inhibitor. Expression levels were assessed 48 hr post-transfection by quantitative RT-PCR and normalised to expression of 18S. Expression of DDAH1 mRNA following miR-193b inhibitor transfection is expressed relative to the NC inhibitor transfection, set to a value of 1. (B) DDAH1 protein expression was assessed by western blotting 72 hr post-transfection. ß-actin expression was used to normalise for total protein. (C) Quantification of DDAH1 protein expression was conducted from three independent experiments and normalised to ß-actin expression. Data are shown relative to DDAH1 expression in NC condition, which is set to a value of 1. All data are the average of at least two independent experiments performed in triplicate. Error bars represent SEM. **p < 0.01 and ***p < 0.001.