Table 1.
Hematological responses to anesthesia and air exposure experiment in tilapia.
| Treatment | Cortisol (nmol/L) | Glucose (mmol/L) | Lactate (mmol/L) | AST (U/L) | ALT (U/L) | RBC (1012 cells/L) | HCT (%) | |
|---|---|---|---|---|---|---|---|---|
| Anesthesia experiment | 10 min anesthesia | 73.3 ± 8.4d | 8.5 ± 0.5d | 1.2 ± 0.2d | ND | ND | ND | 33.7 ± 1.2b |
| 30 min recovery | 104.3 ± 14.7b | 14.9 ± 1.4b | 2.4 ± 0.3bc | ND | ND | ND | 31.7 ± 1.5bc | |
| 90 min recovery | 47.2 ± 6.4e | 8.9 ± 0.5d | 2.7 ± 0.5ab | ND | ND | ND | 30.0 ± 1.0bc | |
| Rest | 53.5 ± 7.3e | 9.5 ± 1.6d | 0.9 ± 0.3d | 82.0 ± 11.0b | 33.0 ± 10.8b | 2.8 ± 0.1b | 28.5 ± 1.0 cd | |
| Air exposure experiment | CO | 77.1 ± 10.7 cd | 14.7 ± 1.3b | 2.0 ± 1.7bcd | 105.3 ± 8.0b | 30.7 ± 10.8b | 2.3 ± 0.6b | 25.3 ± 6.4d |
| CO&AN&CT | 93.0 ± 6.6bc | 12.8 ± 0.5bc | 0.6 ± 0.3d | 114.3 ± 12.1b | 41.3 ± 16.3b | 2.8 ± 0.1b | 31.3 ± 1.2bc | |
| CO&AN&AE | 186.0 ± 8.6a | 21.6 ± 0.9a | 4.7 ± 2.0a | 224.3 ± 23.7a | 128.0 ± 30.0a | 3.6 ± 0.1a | 39.7 ± 2.3a | |
10 min anesthesia, 30 min recovery and 90 min recovery refer to the anesthesia experiment; CO, CO&AN&CT and CO&AN&AE refer to the air exposure experiment. CO = cooling, CO&AN&CT = cooling, anesthesia and cold tolerance control, CO&AN&AE = cooling, anesthesia and air exposure treatment, ND = not detected. Values are means ± SD (n = 10, 5 per replicate). Different letters indicate significant differences (P < 0.05) between treatments. Rest values were taken from fish without any treatment (30 °C).