Figure 4.
MDSCs from CebpaΔ/Δ mice exhibit enhanced activities in tumor angiogenesis and tumor growth. WT and CebpaΔ/Δ mice were injected with 1 × 105 3LL or B16 tumor cells in the flank. After 21 days, Gr-1+CD11b+ cells were purified from the spleens by FACS. WT C57BL/6 mice were injected with 5 × 105 3LL or B16 tumor cells alone, 3LL or B16 cells combined with 5 × 104 Gr-1+CD11b+ cells from CebpaΔ/Δ mice or littermate WT controls. Tumor dimensions were measured every 2–3 days with a caliper and tumor volume was calculated and plotted with time (A and B). n = 10 mice per group and repeated once. Tumors were harvested, sectioned and stained with CD31 antibody. The number of CD31+ structures was quantified in randomly selected fields under microscopy of 3LL tumors (C) and B16 tumors. *p < 0.05. The data are presented as mean with SD.